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A systematic survey of LU domain-containing proteins reveals a novel human gene, LY6A, which encodes
《医学前沿(英文)》 2023年 第17卷 第3期 页码 458-475 doi: 10.1007/s11684-022-0968-4
关键词: LU domain-containing protein family novel human gene LY6A pituitary tumor biomarker nonsynonymous SNP GPI-anchored protein
The antibiotic resistome: gene flow in environments, animals and human beings
null
《医学前沿(英文)》 2017年 第11卷 第2期 页码 161-168 doi: 10.1007/s11684-017-0531-x
The antibiotic resistance is natural in bacteria and predates the human use of antibiotics. Numerous antibiotic resistance genes (ARGs) have been discovered to confer resistance to a wide range of antibiotics. The ARGs in natural environments are highly integrated and tightly regulated in specific bacterial metabolic networks. However, the antibiotic selection pressure conferred by the use of antibiotics in both human medicine and agriculture practice leads to a significant increase of antibiotic resistance and a steady accumulation of ARGs in bacteria. In this review, we summarized, with an emphasis on an ecological point of view, the important research progress regarding the collective ARGs (antibiotic resistome) in bacterial communities of natural environments, human and animals, i.e., in the one health settings. We propose that the resistance gene flow in nature is “from the natural environments” and “to the natural environments”; human and animals, as intermediate recipients and disseminators, contribute greatly to such a resistance gene “circulation.”
null
《医学前沿(英文)》 2013年 第7卷 第3期 页码 280-289 doi: 10.1007/s11684-013-0265-3
Many gene fusions have been recognized as important diagnostic and/or prognostic markers in human malignancies. In recent years, novel gene fusions have been identified in cases without prior knowledge of the genetic background. Accompanied by a powerful computational data analysis method, new genome-wide screening approaches were used to detect cryptic genomic aberrations. This review focused on advanced genome-wide screening approaches in fusion gene identification, such as microarray-based approaches, next-generation sequencing, and NanoString nCounter gene expression system. The fundamental rationale and strategy for fusion gene identification using each biotech platform are also discussed.
关键词: gene fusion cancer microarray next-generation sequencing NanoString nCounter system
A novel variant in the gene in a large Chinese family with a unique phenotype of Clouston syndrome
《医学前沿(英文)》 2023年 第17卷 第2期 页码 330-338 doi: 10.1007/s11684-022-0933-2
关键词: Clouston syndrome whole exome sequencing GJB6 gene novel variant unique phenotype
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
null
《医学前沿(英文)》 2015年 第9卷 第1期 页码 90-99 doi: 10.1007/s11684-015-0390-2
Gene therapy provides a potential cure for hemophilia B, and significant progress has been achieved in liver-directed gene transfer mediated by adeno-associated viral vectors. Recent clinical trials involving the use of a self-complementary adeno-associated virus serotype 8-human codon-optimized factor IX (AAV8-hFIXco) vector demonstrated encouraging efficacy with hFIX expression stabilized at 1% to 6% of normal level in patients, but safety concerns related to high vector doses are still present. Thus, further improvement of AAV vectors and hFIX expression cassette may positively contribute to the ultimate success of hemophilia B gene therapy. In this study, to obtain a higher expression level of hFIX that potentiates the coagulant capacity of recipients, human FIX expression vector was optimized by upgrading the codon adaption index and adjusting the GC content, inserting a Kozak sequence (GCCACC), and introducing a gain-of-function mutation, R338L (FIX Padua). The efficiency of the published and the presently constructed cassettes was compared through in vivo screening. In addition, the regulatory elements that control the FIX gene expression in these cassettes were screened for liver-specific effectiveness. Among all the constructed cassettes, scAAV-Pre-hFIXco-SIH-R338L, which was the construct under the control of the prothrombin enhancer and prealbumin promoter, resulted in the highest level of coagulant activity, and the expression levels of two constructed cassettes (scAAV-Chi-hFIXco-SIH-R338L and scAAV-Pre-hFIXco-SIH-R338L) were also higher than that of the published cassette (scAAV-LP1-hFIXco-SJ). In summary, our strategies led to a substantial increase in hFIX expression at the protein level or a remarkably elevated coagulant activity. Thus, these reconstructs of hFIX with AAV vector may potentially contribute to the creation of an efficacious gene therapy of hemophilia B.
关键词: factor IX hemophilia B liver-specific regulatory elements hydrodynamic gene transfer
Antitumor immunity of human SART3 gene vaccine against mouse tumor
HE Yu, YANG Shuhua, LIU Yong, LI Tao
《医学前沿(英文)》 2008年 第2卷 第1期 页码 51-57 doi: 10.1007/s11684-008-0010-5
关键词: antitumor therapy occurrence implantation DNA vaccine SART3 DNA
Compiling of comprehensive data of human infections with novel influenza A (H7N9) virus
null
《医学前沿(英文)》 2013年 第7卷 第3期 页码 275-276 doi: 10.1007/s11684-013-0285-z
a novel K+ transporter gene in cotton
Yiru WANG, Juan XU, Mingcai ZHANG, Xiaoli TIAN, Zhaohu LI
《农业科学与工程前沿(英文)》 2018年 第5卷 第2期 页码 226-235 doi: 10.15302/J-FASE-2017170
A novel aldo-keto reductase gene,
Jinxi HUO, Bing DU, Sifan SUN, Shaozhen HE, Ning ZHAO, Qingchang LIU, Hong ZHAI
《农业科学与工程前沿(英文)》 2018年 第5卷 第2期 页码 206-213 doi: 10.15302/J-FASE-2018225
夏家辉
《中国工程科学》 2000年 第2卷 第11期 页码 1-11
介绍了中国医学遗传学国家重点实验室在遗传病家系收集、疾病基因定位、疾病基因克隆和疾病基因功能研究方面的研究工作。用细胞遗传学G显带技术于1975年发现了一条与鼻咽癌相关的标记染色体t(1;3)(q44;p11);1981年将睾丸决定基因(TDF)定位于Yp11.32带;1991年以来收集遗传病家系345种共590个;1996年用显微切割、PCR、微克隆技术克隆了EXT2基因;1998年用基因家族-候选疾病基因克隆方法克隆了遗传性神经性耳聋基因GJB3;1999年用连锁分析和全基因组扫描将一种遗传性弥漫性浅表性光敏性汗孔角化症定位于12q23.2带,并在基因功能研究中发现了一个新的细胞内转运蛋白。
关键词: 遗传病家系 基因定位和克隆 基因家族-候选疾病基因克隆 基因组扫描 基因功能研究
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
《医学前沿(英文)》 2008年 第2卷 第3期 页码 283-285 doi: 10.1007/s11684-008-0053-7
关键词: D-HPLC mutation development autoimmune PCR-RFLP candidate
Expression and bioinformatic analysis of lymphoma-associated novel gene KIAA0372
BAI Xiangyang, TANG Duozhuang, ZHU Tao, SUN Lishi, YAN Lingling, LU Yunping, ZHOU Jianfeng, MA Ding
《医学前沿(英文)》 2007年 第1卷 第1期 页码 93-98 doi: 10.1007/s11684-007-0018-2
关键词: bioinformatic analysis functional KIAA0372 detection Microarray
Genetic Study Identifies CBLN4 as a Novel Susceptibility Gene for Accident Proneness
Shu-lin Zhang,Hui-qing Jin,Yang Song,Wan-sheng Yu,Liang-dan Sun
《工程管理前沿(英文)》 2016年 第3卷 第1期 页码 30-38 doi: 10.15302/J-FEM-2016008
关键词: accident proneness genome-wide association study (GWAS) dopamine (DA) ACTH susceptibility gene traffic accident epidemiology accident prevention traffic safety three-dimensional model
Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN
《医学前沿(英文)》 2009年 第3卷 第2期 页码 148-152 doi: 10.1007/s11684-009-0032-7
关键词: gene X-linked inhibitor of apoptosis protein associated factor-1 (XAF1) promoter transcription regulation
《医学前沿(英文)》 2022年 第16卷 第4期 页码 584-595 doi: 10.1007/s11684-021-0844-7
关键词: hemophilia A adeno-associated virus (AAV) human/rat hybrid factor VIII gene therapy dual chain strategy
标题 作者 时间 类型 操作
A systematic survey of LU domain-containing proteins reveals a novel human gene, LY6A, which encodes
期刊论文
Identification of cancer gene fusions based on advanced analysis of the human genome or transcriptome
null
期刊论文
A novel variant in the gene in a large Chinese family with a unique phenotype of Clouston syndrome
期刊论文
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
null
期刊论文
Antitumor immunity of human SART3 gene vaccine against mouse tumor
HE Yu, YANG Shuhua, LIU Yong, LI Tao
期刊论文
a novel K+ transporter gene in cotton
Yiru WANG, Juan XU, Mingcai ZHANG, Xiaoli TIAN, Zhaohu LI
期刊论文
A novel aldo-keto reductase gene,
Jinxi HUO, Bing DU, Sifan SUN, Shaozhen HE, Ning ZHAO, Qingchang LIU, Hong ZHAI
期刊论文
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
期刊论文
Expression and bioinformatic analysis of lymphoma-associated novel gene KIAA0372
BAI Xiangyang, TANG Duozhuang, ZHU Tao, SUN Lishi, YAN Lingling, LU Yunping, ZHOU Jianfeng, MA Ding
期刊论文
Genetic Study Identifies CBLN4 as a Novel Susceptibility Gene for Accident Proneness
Shu-lin Zhang,Hui-qing Jin,Yang Song,Wan-sheng Yu,Liang-dan Sun
期刊论文
Cloning of human XAF1 gene promoter and assay of its transcription activity in a variety of cell lines
Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN
期刊论文